Porter JF and Wardlaw AC (1993)
Long-term survival of Bordetella bronchiseptica in lakewater and in buffered saline without added nutrients FEMS Microbiol Lett 110:33-36
Abstract:
Bordetella bronchiseptica grew from small inocula, and retained viability for at least 24 weeks, in unsupplemented
lakewater or phosphate-buffered saline. From washed inocula of around 10(3) colony-forming units/ml, there was growth at
both 10 degrees C and 37 degrees C to give 10(6)-10(7) colony-forming units/ml. At 10 degrees C, these counts were
maintained with little diminution up to week 24 when observations ceased. In the tests at 37 degrees C, two of three
strains tested showed similar retention of viability. These results suggest that B. bronchiseptica may exist as
hitherto unsuspected reservoirs of infection in freshwater habitats.